MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03667cam a2200349 i 4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
0000070288 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
0001 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
161114s2016 nyua b 001 0 eng |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| International Standard Book Number |
9781621821304 (hardcover : alk. paper) |
|
|---|
| International Standard Book Number |
9781621821311 (paperback) |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
DNLM/DLC |
| Transcribing agency |
DLC |
| Description conventions |
rda |
| Modifying agency |
DLC |
| 042 ## - AUTHENTICATION CODE |
|---|
| Authentication code |
pcc |
| 082 00 - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
572.88078 |
| Edition number |
23 |
| 084 ## - OTHER CLASSIFICATION NUMBER |
|---|
| Classification number |
572.88078 |
| Item number |
CRI |
| 245 00 - TITLE STATEMENT |
|---|
| Title |
CRISPR-Cas : |
| Medium |
[Book] : |
| Remainder of title |
a laboratory manual / |
| Statement of responsibility, etc. |
edited by Jennifer Doudna, University of California, Berkeley, Prashant Mali, University of California, San Diego. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
x, 181 pages : |
| Other physical details |
illustrations (some color) ; |
| Dimensions |
28 cm. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before. Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system--especially for minimizing off-target effects--are also provided. Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits. |
| 521 ## - TARGET AUDIENCE NOTE |
|---|
| Target audience note |
All. |
| 521 ## - TARGET AUDIENCE NOTE |
|---|
| Target audience note |
All. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
CRISPR-Cas Systems. |
|
|---|
| Topical term or geographic name entry element |
CRISPR-Associated Proteins. |
|
|---|
| Topical term or geographic name entry element |
Genetic Techniques. |
|
|---|
| Topical term or geographic name entry element |
Laboratory Manuals |
| Geographic subdivision |
RNA--Analysis. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Static relays. |
|
|---|
| Topical term or geographic name entry element |
Voltage regulators. |
|
|---|
| Topical term or geographic name entry element |
Electric power systems. |
| Geographic subdivision |
Engineering. |
|
|---|
| Topical term or geographic name entry element |
Systems engineering. |
| Geographic subdivision |
Engineering economy |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Doudna, Jennifer A., |
| Relator term |
editor. |
|
|---|
| Personal name |
Mali, Prashant, |
| 852 ## - LOCATION |
|---|
| Piece designation |
55143 |
| -- |
12311.64 |
| Classification part |
572.88078 CRI |
| -- |
Pak American Commercial (Pvt.) Ltd. |
| Sublocation or collection |
Ground Floor |
| Former shelving location |
Books |
| Copy number |
1 |
| Piece physical condition |
1-New |
| Location |
JZL-CUI |
| 852 ## - LOCATION |
|---|
| Piece designation |
55581 |
| -- |
12554.44 |
| Classification part |
621.381044 SHA-S |
| -- |
Pak American Commercial (Pvt.) Ltd. |
| Sublocation or collection |
2nd Floor |
| Former shelving location |
Books |
| Copy number |
1 |
| Piece physical condition |
1-New |
| Location |
JZL-CUI |
